Stimulated emission depletion (STED) microscopy is one of the family of super-resolution techniques developed to allow imaging beyond the normal diffraction limit of optical microscopy. The principle ...

But around 1994, in a wonderful lesson teaching us that we must take care when applying fundamental physical principles, Stefan Hell discovered Stimulated Emission Depletion (STED) microscopy, which ...

Nanoscopy describes the ability to see beyond the generally accepted optical limit of 200–300 nm. Stimulated emission depletion (STED) microscopy, developed by Stefan W. Hell and Jan Wichmann in 1994, ...

Novel developments also focus on quick and high-speed AFM to improve temporal resolution. 2 The combination of AFM with optical microscopy has expanded the number of possible applications, ...

Fluorescence light microscopy techniques offer several advantages when imaging biological samples, including high image contrast, good labeling specificity, multicolor, and three dimensional (3D) ...