In addition, the increased sensitivity of the nested, competitive RT-PCR was readily apparent in patients with minimal residual disease, which by the real-time were negative in the majority of ...

The use of new powerful nested polymerase chain reaction (PCR) techniques to identify and screen for prevalence of parasites has a huge potential. It allows for the detection and identification of low ...

Two nested quantitative real-time PCR (qPCR) assays were developed that target the human EPO (hEPO) cDNA sequence in a circular form, representative of recombinant adeno-associated viral (rAAV ...

We sought to determine whether the profiles of gene hypermethylation differ between IBC and non-IBC by using a multiplex nested methylation-specific PCR (MSP). We selected genes for which evidence ...

Nested PCR and multiplex PCR assays provided fast and reliable detection of the major soft rot and blackleg potato pathogens using three pairs of primers (Df/Dr, Y45/46 and EXPCCF/EXPCCR) and enabled ...

Purpose: To determine whether reverse transcriptase polymerase chain reaction (RT-PCR) to detect circulating prostate-specific antigen (PSA)-positive cells is a prognostic factor for survival in ...

Other methods include reduction in the PCR volume, increase in PCR cycle number, nested PCR, amplifying the whole genome before PCR, removing ions after the amplification step, or increasing the ...

PCR-Machines are classified depending on the capacity of PCR used such as Nested PCR or Reverse Transcriptase PCR (RT-PCR ), or Multiplex RT PCR.