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12:53
jove.com
Video: Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
31.6K Views. University of Cambridge. The overall goal of this procedure is to identify protein interaction partners for a given protein of interest. This is accomplished by first growing the cells in cell culture media containing arginine and lysine labeled with different stable isotopes of carbon and nitrogen. The second step is to transfect ...
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